Ginseng: Cardiotonic in adult rat cardiomyocytes, cardiotoxic in neonatal rat cardiomyocytes. Poindexter BJ(1), Allison AW, Bick RJ, Dasgupta A. Author information: (1)Department of Pathology and Laboratory Medicine, University of Texas Medical School at Houston, MSB , Fannin Street, Houston, Texas , USA. This manuscript presents a protocol for successful isolation and cultivation of adult rat ventricular cardiomyocytes (ARVC). The rat is sacrificed by cervical dislocation under deep anesthesia. Then, the heart is extracted and the aorta is uncovered.
Summary Isolation of high-quantity and high-quality ventricular cardiomyocytes from adult rats is critical to study heart physiology and pathology and for drug toxicity screening. It remains challenging to produce a high yield of viable cardiomyocytes from rats. Preparation of adult rat cardiomyocytes Adult rat (Sprague–Dawley; approx. g, 2–4 months) cardiomyocytes are harvested as previously described (Poindexter et al., , Bick et al., ) and used immediately. The heart is exposed by a longitudinal thoracotomy incision and IU of heparin are injected IV.
October 19th, Here, we present a protocol for the isolation and cultivation of adult rat ventricular cardiomyocytes (ARVC). Isolated ARVC can be used for short and long-term cultivation. The isolation and cultivation of ARVC can play a key role in developing new treatment regimens for cardiac diseases. Isolated adult cardiac myocytes maintained in primary culture have been used as a model of the adult myocardium for 20 years. With the recent advances and current interest in using molecular biological techniques to investigate cardiac physiology, culturing myocytes is becoming an increasingly important technique.