Transformation of adult rat cardiac myocytes in primary culture. Banyasz T(1), Lozinskiy I, Payne CE, Edelmann S, Norton B, Chen B, Chen-Izu Y, Izu LT, Balke CW. Author information: (1)University of Kentucky, South Limestone Street, Lexington, KY , USA. [email protected] A method is described for the preparation of Ca 2+-resistant myocytes from the adult bottomed.xyz 2+-resistant cells were obtained in yields of ± × 10 6 cells/gm wet weight tissue, with a purity of ± % cells excluding trypan blue. Of the cells which excluded trypan blue, over 90% were rod-shaped. The rodshaped cells when isolated were quiescent, but beat in response to .
The systolic and diastolic properties of single myocytes and intact papillary muscles isolated from hearts of adult rats and rabbits were examined at 37 degrees C over a range of stimulation frequencies and bathing [Ca2+]o (Cao). In both rabbit myocytes. Calcium-tolerant adult rat ventricular myocytes (ARVMs) were obtained from hearts of male Sprague-Dawley rats ( to g) as previously described. 18 Briefly, animals were anesthetized with sodium pentobarbital (50 mg/kg IP) and heparinized ( USP/kg IV), and their hearts were aseptically removed into an ice-cold modified cardioplegic solution (KB .
Cardiac myocytes isolated from adult rat hearts were grown on laminin coated culture dishes for more than a month. During this time, the cells underwent a morphological transformation which has also been referred to by others as cell remodeling (Guo J-X, Jacobson SL, Brown DL: Cell Mot Cytoskeleton ; –). This results in a change in myocyte morphology from its . Structural and functional properties of adult rat heart myocytes lysed with digitonin. J Biol Chem. Nov 15; (26)– Wenger WC, Murphy MP, Kindig OR, Capen CC, Brierley GP, Altschuld RA. Mitochondrial enzyme retention by irreversibly damaged rectangular isolated adult rat heart myocytes.